Effect of microRNA-133b on Myocardial Fibrosis / 中国医学科学院学报

Objective To investigate the effect of microRNA-133b(miR-133b)on cardiac fibrosis and its mechanism.Methods Human cardiac fibroblasts(CFs)were harvested.The proliferation of CFs was detected by CCK8 during the overexpression and knock-down of miR-133b.The expressions of connective tissue growth factor(CTGF),α-smooth muscle actin(α-SMA),collagen Ⅰ,and collagen Ⅲ were detected with qRT-PCR and Western blot analysis after miR-133b overexpression or downexpression.Target genes of miR-133b were predicted by bioinformatics software.Dual-luciferase activity assay were used to verify a target gene of miR-133b.Results qRT-PCR showed that the expression level of miR-133b in the miR-133b mimic group was significantly higher than that in the negative control group(=26.219,=0.000).The expression level of miR-133b in the miR-133b inhibitor group was significantly lower than that in the negative control group(=6.738,=0.003).After 21,45,69,93,and 117 hours of transfection,the proliferation ability of CFs significantly decreased in the miR-133b mimic group but significantly increased in the miR-133b group(all <0.05,compared with the negative control group).After overexpression of miR-133b,the mRNA and protein levels of CTGF(=9.213,=0.001;=8.195,=0.001),α-SMA(=6.511, =0.003;=4.434,=0.011),collagenⅠ(=3.172,=0.034;=4.053,=0.015)and collagen Ⅲ(=6.404,=0.003;=5.319,=0.006)were significantly down-regulated.After the expression of miR-133b was knocked down,the mRNA and protein levels of CTGF(=9.439,=0.001;=14.100,=0.000),α-SMA(=4.519,=0.011;=4.377,=0.012),collagen Ⅰ(=5.966,=0.004;=5.514,=0.005)and collagen Ⅲ(=4.622,=0.010;=4.996,=0.008)were significantly increased.The relative luciferase activity of the cells co-transfected with miR-133b mimic and WT 3'UTR expression vector was significantly lower than that of the cells co-transfected with mimic control and WT 3'UTR expression vectors(=5.654,=0.005);however,there was no significant difference in relative luciferase activity between cells co-transfected with miR-133b mimic and MUT 3'UTR expression vectors and cells co-transfected with mimic control and MUT 3'UTR expression vectors(=0.380,=0.724).Conclusion miR-133b may affect the activation and proliferation of CFs by targeting CTGF and thus improve cardiac fibrosis.

Effects of up regulation of GDF-15 expression on biological characteristics and PI3K/AKT signaling pathway in H9C2 cardiomyocytes induced by H2O2 / 中国免疫学杂志

Objective:To investigate the effect of up regulation of GDF-15 expression on the proliferation,apoptosis and PI3K/AKT signaling pathway of H9C2 cardiomyocytes induced by H2O2.Methods:CCK8 method was used to detect the proliferation of H9C2 cardiomyocytes treated with different concentrations of H2O2;H9C2 cells were divided into Control group,NC group,H2O2group,GDF-15+H2O2group,the cells were treated for 24 h,mRNA and protein expression of GDF-15 in each group were detected by RT-PCR and Western blot;the proliferation and apoptosis of the cells were detected by CCK8 and flow cytometry respectively;DCFH-DA probe was used to detect the level of ROS;the expression of Ki67,Bcl-2,Bax,PI3K and p-AKT protein was detected by Western blot.H9C2 cells were treated with 10 μmol/L LY294002(a PI3K/AKT signal pathway inhibitor),cell viability and apoptosis rate were detected by CCK8 assay and flow cytometryin espectively.Ki67,Bcl-2,Bax,PI3K and p-AKT protein expression were detected by Western blot.Results:Cell viability was inhibited after different concentrations H2O2treated H9C2 myocardial cells,which was concentration de-pendent (P<0.05),due to H9C2 cardiomyocytes treated with 200 μmol/L H2O2inhibited nearly half of cell proliferation,and were chosen as subjects.Compared with control group,mRNA and protein expression of GDF-15 in H2O2group were significantly increased, cell proliferation was decreased significantly,the apoptosis rate was increased,ROS level was increased,the expression of Ki67,Bcl-2, PI3K,p-AKT protein were decreased,Bax protein expression was increased(P<0.05).Compared with H2O2group,mRNA and protein expression of GDF-15 in GDF-15+H2O2group were significantly increased,cell proliferation was significantly increased,the apoptosis rate was decreased,ROS level was decreased,the expression of Ki67,Bcl-2,PI3K,p-AKT protein were increased,and Bax protein expression was decreased (P<0.05).The cell viability and protein expression of Bcl-2,PI3K and p-AKT in PI3K/AKT inhibitor group were significantly lower than those in GDF-15+H2O2group,and the apoptosis rate and Bax protein expression were significantly higher than those in GDF-15+H2O2group(P<0.05).Conclusion:Up regulation of GDF-15 expression promote the proliferation of H9C2 car-diomyocytes induced by H2O2and reduce apoptosis,and the mechanism may be related to the regulation of ROS levels,Ki67,Bcl-2,Bax expression and PI3K/AKT signaling pathway in cells.

Effect of neuregulin-1 on cardiomyocyte apoptosis and oxidative damage under hypoxia reoxygenation / 中国免疫学杂志

Objective:To investigate the effect of NRG-1 on cardiomyocyte apoptosis and oxidative damage under hypoxia reox-ygenation.Methods:The myocardial cell HCM was taken as the object of study.The hypoxia reoxygenation of myocardial cell model was established,and NRG-1 at dose of 0.8 mg/L was added before hypoxia.The cell viability was measured by MTT assay and apoptosis was analyzed by flow cytometry.The level of lactate dehydrogenase(LDH) in the supernatant was detected by 2,4-dinitrophe-nylhydrazine colorimetry assay,DCFH-DA method was used to detected ROS level,the level of MDA was measured by thiobarbituric acid method,the level of SOD was detected by xanthine oxidase method,and the protein levels of Akt and p-AktThr308were determined by Western blot.Results:The A values of the myocardial cells after hypoxia reoxygenation were changed from 0.66±0.03 to 0.36±0.04, the rate of apoptosis increased from (4.62±0.97)% to (29.07±3.43)%,the level of ROS increased from 69.29±7.96 to 280.84± 20.52,the levels of LDH and MDA also increased,and the levels of SOD and p-AktThr308/Akt decreased.After NRG-1 treatment,the A values of the cells were from 0.36±0.04 to 0.47±0.05,the rate of apoptosis decreased from (29.07±3.43)% to (19.76±3.41)%, the ROS levels decreased from 280.84±20.52 to 128.23±12.32,the levels of LDH and MDA also decreased,and the levels of SOD and p-Akt/Akt increased.Conclusion: NRG-1partly inhibits cardiomyocyte apoptosis and oxidative damage induced by hypoxia reoxygenation by affecting the protein levels of p-Akt/Akt in the cardiomyocytes.

Alkaline and neutral multi enzyme cleaning agent is reusable bone needle cleaning effect / 中国生化药物杂志

Objective To compare the effect of alkaline and neutral multi-enzyme cleanser cleaning and reuse of bone through the needle. Methods 200 pollution recycling reusable bone needle, were randomly divided into two groups. The control group was washed with neutral multienzyme detergent, the ratio was 1/270. The experimental group was cleaned by alkaline multienzyme detergent, with a ratio of 1/270. A comparative analysis of the surface cleaning of the experimental group and control group, the qualified rate of strip inspection pass rate and needle cavity wall cleaning qualified rate and other indicators. Results After cleaning with different cleaning agents, the number of qualified surface cleaning of the experimental group was 98 pieces, and the number of qualified surface cleaning of the control group was 97 pieces, he qualified rate of the surface cleaning of the experimental group and the control group was 98.0% and 97.0%, and there was no statistical difference. In the experimental group, the number of the cleaning of the needle cavity wall was 96, and the number of qualified cleaning of the needle cavity wall was 88. In the control group, the qualified rate of the cleaning of the needle cavity wall was 88.0%, significantly lower than that of the experimental group, and the qualified rate of cleaning was 96.0%, which was statistically significant (P<0.05). The experimental group strip inspection pass rate of 95.0%was significantly higher than the control group of qualified rate was 86.0%, with statistical difference (P<0.05). Conclusion Alkaline multi enzyme cleaning agent compared with neutral multi enzyme cleaning agent, reusable bone needle cleaning effect is more ideal, to ensure the cleaning and sterilization quality, improve the service life.

Alkaline and neutral multi enzyme cleaning agent is reusable bone needle cleaning effect / 中国生化药物杂志

Objective To compare the effect of alkaline and neutral multi-enzyme cleanser cleaning and reuse of bone through the needle. Methods 200 pollution recycling reusable bone needle, were randomly divided into two groups. The control group was washed with neutral multienzyme detergent, the ratio was 1/270. The experimental group was cleaned by alkaline multienzyme detergent, with a ratio of 1/270. A comparative analysis of the surface cleaning of the experimental group and control group, the qualified rate of strip inspection pass rate and needle cavity wall cleaning qualified rate and other indicators. Results After cleaning with different cleaning agents, the number of qualified surface cleaning of the experimental group was 98 pieces, and the number of qualified surface cleaning of the control group was 97 pieces, he qualified rate of the surface cleaning of the experimental group and the control group was 98.0% and 97.0%, and there was no statistical difference. In the experimental group, the number of the cleaning of the needle cavity wall was 96, and the number of qualified cleaning of the needle cavity wall was 88. In the control group, the qualified rate of the cleaning of the needle cavity wall was 88.0%, significantly lower than that of the experimental group, and the qualified rate of cleaning was 96.0%, which was statistically significant (P<0.05). The experimental group strip inspection pass rate of 95.0%was significantly higher than the control group of qualified rate was 86.0%, with statistical difference (P<0.05). Conclusion Alkaline multi enzyme cleaning agent compared with neutral multi enzyme cleaning agent, reusable bone needle cleaning effect is more ideal, to ensure the cleaning and sterilization quality, improve the service life.

Advance in immunology and immune evasion of PRRSV / 病毒学报

Porcine reproductive and respiratory syndrome virus (PRRSV) continues to be a threat, causing economically significant impacts on the swine industry worldwide. Unfortunately, the traditional control strategies and conventional vaccines fail to provide sustainable disease control, in particular against genetically diverse strains, as they suffer from both antigenic heterogeneity and various immune evasion strategies of PRRSV. In this paper, latest research progress in immunology and immune evasion of PRRSVis summarized to provide a referenc for PRSSV prevention and control as well as the design of new vaccines.

Detection of ICAM-1 and RANTES in infants with bronchiolitis / 中国当代儿科杂志

<p><b>OBJECTIVE</b>To explore the role of intercellular adhesion molecule-1 (ICAM-1) and regulated upon activation normal T cell expressed and secreted (RANTES) in bronchiolitis and their correlation in the pathogenesis of this disorder.</p><p><b>METHODS</b>The expression of ICAM-1 was detected by flow cytometry on lymphocytes of peripheral blood in 28 infants with bronchiolitis, 23 infants with bronchopneumonia and 24 healthy infants. Serum level of RANTES was assayed using ELISA. The correlation between ICAM-1 and RANTES levels was evaluated using Pearson correlation coefficient.</p><p><b>RESULTS</b>The ICAM-1 level in the bronchiolitis group (35.0+/-10.3%) was much higher than that in the bronchopneumonia (29.9+/-8.6%; p<0.05) and the control groups (24.6+/-6.9%; p<0.01). The bronchopneumonia group had higher ICAM-1 level than the control group (p<0.05). The RANTES level in the bronchiolitis (32.1+/-6.0 ng/mL) and the bronchopneumonia groups (30.6+/-6.2 ng/mL) was significantly higher than that in the control group (27.1+/-5.1 ng/mL) (p<0.01, p<0.05, respectively), however, no significant difference was found between the bronchopneumonia and bronchiolitis groups. There was a positive correlation between ICAM-1 and RANTES levels in the bronchiolitis group (r=0.675, P<0.01).</p><p><b>CONCLUSIONS</b>ICAM-1 and RANTES are involved in the pathogenesis of bronchiolitis and show a synergistic effect.</p>